rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts-Reference-Cited by-同舟云学术

rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts

Published:2020-05-14 Issue:1 Volume:10 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Sousa Susana,Almeida André,Delgado Lurdes,Conceição Antónia,Marques Cláudia,da Costa José Manuel Correia,Castro António

Abstract

AbstractToxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1–19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/s41598-020-64590-4.pdf

Reference25 articles.

1. Dubey, J.P. & Beattie, C. P. Toxoplasmosis of animals and man. (CRC press, Inc., 1988).

2. Dumètre, A. & Dardé, M. L. How to detect Toxoplasma gondii oocysts in environmental samples? FEMS Microbiol Rev. 27, 651–661 (2003).

3. Dubey, J. P. et al. Prevalence of viable Toxoplasma gondii in beef, chickens, and pork from retail meat stores in the United States: Risk assessment to consumers. J Parasitol. 91, 1082–1093 (2005).

4. Robert-Gangneux, F. & Marie-Laure Dardé, M. L. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 25, 264–296 (2012).

5. Shapiro, K. et al. Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay. Food Microbiol. 84, 103252 (2019).

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