Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia
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Published:2024-06-26
Issue:1
Volume:14
Page:
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ISSN:2045-2322
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Container-title:Scientific Reports
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language:en
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Short-container-title:Sci Rep
Author:
Alhaithloul Haifa A. S.,Awad Nabil S.,Qari Sameer H.,El-Homosy Rania F.,Qaoud El-Sayed M.,Alqahtani Mesfer M.,Ghanem Kholoud Z.,Alasmari Abdulrahman,Alzuaibr Fahad M.,Ghazzawy Hesham S.,Abdein Mohamed A.
Abstract
AbstractIn light of the multitude of olive trees cultivated and the lack of the genetic diversity of available genotypes to select varieties and lines that are characterized by high diversity and better performance under the corresponding conditions, A comparison analysis of the genotyping and morphological characteristics of eight olive cultivars growing in Saudi Arabia’s Al-Jouf region was conducted and analyzed. Morpho-anatomical and chemical characteristics along with both inter-simple-sequence repeats (ISSRs) and start-codon-targeted (SCoT) markers were used to evaluate the genetic diversity among eight olive varieties in Al-Jouf, Saudi Arabia. Analyses of 27 morphological, chemical, and anatomical characteristics concluded the existence of genetic differences among the studied varieties. Moreover, six ISSR and eight SCoT primer combinations produced a total of 48 loci, of which 18 (10 ISSR and 8 SCoT) were polymorphic. The average polymorphism information content (PIC values of 0.48 and 0.44, respectively) and marker index (MI of 0.79 and 0.48, respectively) detected for ISSR and SCoT markers revealed the prevalence of high genetic diversity among the studied olive varieties. Based on chemical and anatomical characteristics and the selected molecular markers, the eight olive cultivars were grouped into two distinct clusters. Clusters in the adjacent joint dendrogram produced using ISSR, SCoT and combined data were similar, and grouped all individuals into two groups. However, the dendrogram generated on the basis of SCoT separated individuals into subgroups containing at least two varieties. The findings showed that both methods were effective in assessing diversity, and that SCoT markers can be used as a reliable and informative method for assessing genetic diversity and relationships among olive varieties and can serve as a complementary tool to provide a more complete understanding of the genetic diversity available in Olea europaea populations in Saudi Arabia.
Funder
Deanship of Scientific Research at Jouf University funded this work through research
Publisher
Springer Science and Business Media LLC
Reference76 articles.
1. Hassouna, V. Virgin olive oil 23 (Demand GmbH, Norderstedt, Germany, 2010).
2. FAOSTAT. Food and agriculture organization of United Nations. (2014).
3. Barranco, D. & Rallo, L. Olive cultivars in Spain. HortTechnology 10(1), 107–110 (2000).
4. Bartolini, G. et al. Olive germplasm: Cultivars and world-wide collections (FAO, Rome, Italy, 1998).
5. Guerfel, M. et al. Effect of location on virgin olive oils of the two main Tunisian olive cultivars. Eur. J. Lipid Sci. Technol. 111(9), 926–932 (2009).