Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy

Author:

Martinez-Vidal Laura,Testi Claudia,Pontecorvo Emanuele,Pederzoli Filippo,Alchera Elisa,Locatelli Irene,Venegoni Chiara,Spinelli Antonello,Lucianò Roberta,Salonia Andrea,Podestà Alessandro,Ruocco Giancarlo,Alfano Massimo

Abstract

AbstractBladder mechanical properties are critical for organ function and tissue homeostasis. Therefore, alterations of tissue mechanics are linked to disease onset and progression. This study aims to characterize the tissue elasticity of the murine bladder wall considering its different anatomical components, both in healthy conditions and in actinic cystitis, a state characterized by tissue fibrosis. Here, we exploit Brillouin microscopy, an emerging technique in the mechanobiology field that allows mapping tissue mechanics at the microscale, in non-contact mode and free of labeling. We show that Brillouin imaging of bladder tissues is able to recognize the different anatomical components of the bladder wall, confirmed by histopathological analysis, showing different tissue mechanical properties of the physiological bladder, as well as a significant alteration in the presence of tissue fibrosis. Our results point out the potential use of Brillouin imaging on clinically relevant samples as a complementary technique to histopathological analysis, deciphering complex mechanical alteration of each tissue layer of an organ that strongly relies on mechanical properties to perform its function.

Funder

European Union’s Horizon 2020 Marie Skłodowska-Curie Action

European Union’s Horizon 2020

European Research Council

European Innovation Council

Publisher

Springer Science and Business Media LLC

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