Author:
Donnelley Martin,Cmielewski Patricia,Morgan Kaye,Delhove Juliette,Reyne Nicole,McCarron Alexandra,Rout-Pitt Nathan,Drysdale Victoria,Carpentieri Chantelle,Spiers Kathryn,Takeuchi Akihisa,Uesugi Kentaro,Yagi Naoto,Parsons David
Abstract
AbstractGene vectors to treat cystic fibrosis lung disease should be targeted to the conducting airways, as peripheral lung transduction does not offer therapeutic benefit. Viral transduction efficiency is directly related to the vector residence time. However, delivered fluids such as gene vectors naturally spread to the alveoli during inspiration, and therapeutic particles of any form are rapidly cleared via mucociliary transit. Extending gene vector residence time within the conducting airways is important, but hard to achieve. Gene vector conjugated magnetic particles that can be guided to the conducting airway surfaces could improve regional targeting. Due to the challenges of in-vivo visualisation, the behaviour of such small magnetic particles on the airway surface in the presence of an applied magnetic field is poorly understood. The aim of this study was to use synchrotron imaging to visualise the in-vivo motion of a range of magnetic particles in the trachea of anaesthetised rats to examine the dynamics and patterns of individual and bulk particle behaviour in-vivo. We also then assessed whether lentiviral-magnetic particle delivery in the presence of a magnetic field increases transduction efficiency in the rat trachea. Synchrotron X-ray imaging revealed the behaviour of magnetic particles in stationary and moving magnetic fields, both in-vitro and in-vivo. Particles could not easily be dragged along the live airway surface with the magnet, but during delivery deposition was focussed within the field of view where the magnetic field was the strongest. Transduction efficiency was also improved six-fold when the lentiviral-magnetic particles were delivered in the presence of a magnetic field. Together these results show that lentiviral-magnetic particles and magnetic fields may be a valuable approach for improving gene vector targeting and increasing transduction levels in the conducting airways in-vivo.
Funder
National Health and Medical Research Council
Cystic Fibrosis Foundation
Australian Research Council
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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1. Noncontact Respiratory Motion Detection in Anesthetized Rodents;Journal of the American Association for Laboratory Animal Science;2023-11-11