Author:
Wang Hong-Ping,Lin Zhao-Zhou,Wang Hui,Yang Xuan,Niu Nan
Abstract
AbstractCitri Reticulatae Pericarpium is a traditional Chinese medicine with extremely high health benefits as well as clinical value. In vivo and in vitro tests have proved that its main active secondary metabolites are flavonoids. However, they have not been comprehensively analyzed up to now mainly due to lack of suitable analysis method. To solve this problem, a novel strategy based on precursor ions locked and targeted MS/MS analysis was proposed. Firstly, the database of the flavonoids previously isolated from Citri Reticulatae Pericarpium was established to obtain the characteristics of their precursor ions. Secondly, after performing the full MS scan of the extract, all compounds in the total ion chromatogram were extracted by Compound Discoverer software. Thirdly, the precursor ions of the flavonoids were locked from the extracted compounds according to their characteristics, forming a precursor ions list. Finally, the precursor ions in the constructed list were performed targeted MS/MS analysis for structures characterization. As a result, total 187 flavonoids were successfully identified, and except for flavones, flavonols as well as dihydroflavones, some chalcones were also characterized from Citri Reticulatae Pericarpium for the first time.
Publisher
Springer Science and Business Media LLC
Reference32 articles.
1. Zou, J. M. et al. Citri Reticulatae Pericarpium (Chenpi): A multi-efficacy pericarp in treating cardiovascular diseases. Biomed. Pharmacother. 154, 113626 (2022).
2. Li, X. C., Huang, Y. P. & Chen, D. F. Protective effect against hydroxyl-induced DNA damage and antioxidant activity of Citri Reticulatae Pericarpium. Adv. Pharm. Bull. 3, 175–181 (2013).
3. Ma, E. Y. et al. Bioinformatics-guided identification of ethyl acetate extract of Citri Reticulatae Pericarpium as a functional food ingredient with anti-inflammatory potential. Molecules 27, 5435 (2022).
4. Wang, J. et al. Citri Reticulatae Pericarpium-Reynoutria japonica Houtt. herb pair suppresses breast cancer liver metastasis by targeting ECM1-mediated cholesterol biosynthesis pathway. Phytomedicine 116, 154896 (2023).
5. Yu, J. J., Su, J., Yan, M. Q., Lou, Z. H. & Lv, G. Y. Correlation between lipid-lowering efficacy and components of Pericarpium Citri Reticulatae. Chin. J. Chin. Mater. Med. 44, 3335–3342 (2019).