Abstract
AbstractDengue is an important arboviral infection, causing a broad range symptom that varies from life-threatening mild illness to severe clinical manifestations. Recent studies reported the impairment of the central nervous system (CNS) after dengue infection, a characteristic previously considered as atypical and underreported. However, little is known about the neuropathology associated to dengue. Since animal models are important tools for helping to understand the dengue pathogenesis, including neurological damages, the aim of this work was to investigate the effects of intracerebral inoculation of a neuroadapted dengue serotype 2 virus (DENV2) in immunocompetent BALB/c mice, mimicking some aspects of the viral encephalitis. Mice presented neurological morbidity after the 7th day post infection. At the same time, histopathological analysis revealed that DENV2 led to damages in the CNS, such as hemorrhage, reactive gliosis, hyperplastic and hypertrophied microglia, astrocyte proliferation, Purkinje neurons retraction and cellular infiltration around vessels in the pia mater and in neuropil. Viral tropism and replication were detected in resident cells of the brain and cerebellum, such as neurons, astrocyte, microglia and oligodendrocytes. Results suggest that this classical mice model might be useful for analyzing the neurotropic effect of DENV with similarities to what occurs in human.
Publisher
Springer Science and Business Media LLC
Reference63 articles.
1. Bhatt, S. et al. The global distribution and burden of dengue. Nature. 496, 504–507 (2013).
2. Martina, B. E., Koraka, P. & Osterhaus, A. D. Dengue virus pathogenesis: an integrated view. Clin. Microbiol. Rev. 22, 564–581 (2009).
3. WHO, World Health Organization. Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd ed. Geneva, World Health Organization. https://www.who.int/csr/resources/publications/dengue/Denguepublication/en/ (1997).
4. Carod-Artal, F. J., Wichmann, O., Farrar, J. & Gascón, J. Neurological complications of dengue virus infection. Lancet Neurol. 12, 906–919 (2013).
5. Sanguansermsri, T., Poneprasert, B. & Phornphutkul, B. Acute Encephalopathy Associated with Dengue Infection. Bangkok: Seameo Tropmed. 10 (1976).
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