Abstract
AbstractRoot-knot nematode is a very destructive pathogen, to which most peanut cultivars are highly susceptible. Strong resistance is present in the wild diploid peanut relatives. Previously, QTLs controlling nematode resistance were identified on chromosomes A02, A04 and A09 ofArachis stenosperma. Here, to study the inheritance of these resistance alleles within the genetic background of tetraploid peanut, an F2population was developed from a cross between peanut and an induced allotetraploid that incorporatedA. stenosperma, [Arachis batizocoixA. stenosperma]4×. This population was genotyped using a SNP array and phenotyped for nematode resistance. QTL analysis allowed us to verify the major-effect QTL on chromosome A02 and a secondary QTL on A09, each contributing to a percentage reduction in nematode multiplication up to 98.2%. These were validated in selected F2:3lines. The genome location of the large-effect QTL on A02 is rich in genes encoding TIR-NBS-LRR protein domains that are involved in plant defenses. We conclude that the strong resistance to RKN, derived from the diploidA. stenosperma, is transferrable and expressed in tetraploid peanut. Currently it is being used in breeding programs for introgressing a new source of nematode resistance and to widen the genetic basis of agronomically adapted peanut lines.
Publisher
Springer Science and Business Media LLC
Reference69 articles.
1. Bertioli, D. J. et al. The genome sequence of peanut (Arachis hypogaea), a segmental allotetraploid. Nat Genet 51, 877–884, https://doi.org/10.1038/s41588-019-0405-z (2019).
2. Bertioli, D. J. et al. The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut. Nat Genet 48, 438–446, https://doi.org/10.1038/ng.3517 (2016).
3. Halward, T., Stalker, T., LaRue, E. & Kochert, G. Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol 18, 315–325 (1992).
4. Husted, L. Cytological studies on the peanut, Arachis. II. Chromosome number, morphology and behavior, and their application to the problem of the origin of the cultivated forms. Cytologia 7, 396–423 (1936).
5. Kochert, G. et al. RFLP and cytogenetic evidence on the origin and evolution of allotetraploid domesticated peanut, Arachis hypogaea (Leguminosae). Amer J Bot 83, 1281–1291, https://doi.org/10.2307/2446112 (1996).
Cited by
24 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献