Author:
Asanprakit Wichitra,Lobo Dileep N.,Eremin Oleg,Bennett Andrew J.
Abstract
AbstractHigh expression of polymeric immunoglobulin receptor (PIGR) in breast cancer is associated with increased 5-year survival rate. However, the factors influencing PIGR expression in breast cancer have not been elucidated. The aim of this study was to determine the role of macrophages and cytokines affecting expression of PIGR in two breast cancer cell lines. M1, M2 macrophage conditioned media (CM) and recombinant human cytokines were used to determine factors which increased PIGR expression in MCF7 (HTB-22) and MDA-MB468 (HTB-132) breast cancer cell lines. The level of PIGR expression in the cells and PIGR secretory component were evaluated by real-time quantitative polymerase chain reaction and Western blotting. M1 macrophage CM induced a dose-dependent increase in PIGR mRNA expression in MDA-MB468 cells, up to 20-fold. The level of PIGR expression in MCF7 cells was very low and not affected by M1 and M2 CM. Interferon gamma (IFN-γ) and interleukin (IL)-1β also increased PIGR expression in MDA-MB468 and MCF7 cells. However, IL-1β was demonstrated to increase in M1 macrophages, while IFN-γ was not. The role of IL-1β secreted from M1 macrophages in increasing expression of PIGR was confirmed by IL-1 receptor blockade, indicating that IL-1β was the major M1 macrophage-derived cytokine that enhanced PIGR expression. Elevated PIGR expression in breast cancer in vivo may reflect the polarization state of tumor-associated immune cells.
Funder
Royal Thai Army and the Ministry of Higher Education, Science, Research and Innovation, Thailand
Medical Research Council
Arthritis Research UK
FRAME
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Harris, J. P. & South, M. A. Secretory component: A glandular epithelial cell marker. Am. J. Pathol. 105, 47–53 (1981).
2. Kaetzel, C. S. The polymeric immunoglobulin receptor: Bridging innate and adaptive immune responses at mucosal surfaces. Immunol. Rev. 206, 83–99. https://doi.org/10.1111/j.0105-2896.2005.00278.x (2005).
3. Macpherson, A. J., Geuking, M. B., Slack, E., Hapfelmeier, S. & McCoy, K. D. The habitat, double life, citizenship and forgetfulness of IgA. Immunol. Rev. 245, 132–146. https://doi.org/10.1111/j.1600-065X.2011.01072.x (2012).
4. Ai, J. et al. The role of polymeric immunoglobulin receptor in inflammation-induced tumor metastasis of human hepatocellular carcinoma. J. Natl. Cancer. Inst. 103, 1696–1712. https://doi.org/10.1093/jnci/djr360 (2011).
5. Liu, F. et al. Colorectal polymeric immunoglobulin receptor expression is correlated with hepatic metastasis and poor prognosis in colon carcinoma patients with hepatic metastasis. Hepatogastroenterology 61, 652–659 (2014).
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献