Author:
Lukšić Katarina,Zdunić Goran,Hančević Katarina,Mihaljević Maja Žulj,Mucalo Ana,Maul Erika,Riaz Summaira,Pejić Ivan
Abstract
AbstractWild grapevine (Vitis vinifera subsp. sylvestris) is widely recognized as an important source of resistance or tolerance genes for diseases and environmental stresses. Recent studies revealed partial resistance to powdery mildew (Erysiphe necator, PM) in V. sylvestris from Central Asia. Here, we report resistance to PM of V. sylvestris collected from different regions of Croatia and in seedling populations established from in situ V. sylvestris accessions. Ninety-one in situ individuals and 67 V. sylvestris seedlings were evaluated for PM resistance according to OIV 455 descriptor. Three SSR markers (SC47-18, SC8-071-0014, and UDV-124) linked to PM resistance locus Ren1 were used to decipher allelic structure. Nine seedlings showed resistance in in vivo evaluations while leaf disk assays revealed three PM-resistant accessions. One V. vinifera cultivar used as a control for PM evaluations also showed high phenotypic resistance. Based on the presence of one or two resistance alleles that are linked to the Ren1 locus, 32 resistant seedlings and 41 resistant in situ genotypes were identified in the investigated set. Eight seedlings showed consistent phenotypic PM resistance, of which seven carried one or two alleles at the tested markers. This study provides the first evidence of PM resistance present within the eastern Adriatic V. sylvestris germplasm.
Publisher
Springer Science and Business Media LLC
Reference34 articles.
1. Pirrello, C. et al. Emergent Ascomycetes in viticulture: An interdisciplinary overview. Front. Plant Sci. 10, 1394 (2019).
2. Jones, L. et al. Adaptive genomic structural variation in the grape powdery mildew pathogen, Erysiphe necator. BMC Genomics 15, 1081 (2014).
3. European Commission. The Use of Plant Protection Products in the European Union Data 1992–2003. (European Union: Brussels, 2007).
4. Riaz, S., Tenscher, A., Pap, D., Romero, N. & Walker, M. A. Durable powdery mildew resistance in grapevines: Myth or reality. Acta Hortic. 1248, 595–600 (2019).
5. Suthaparan, A. et al. Suppression of powdery mildews by UV-B: Application frequency and timing, dose, reflectance, and automation. Plant Dis. 100, 1643–1650 (2016).
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