Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production
Author:
Funder
National Science Foundation
U.S. Department of Energy
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-019-41759-0.pdf
Reference41 articles.
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3. Bao, Z. et al. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. Nat. Biotechnol. 36, 505–508 (2018).
4. Ross-Macdonald, P., Sheehan, A., Friddle, C., Roeder, G. S. & Snyder, M. Transposon mutagenesis for the analysis of protein production, function, and localization. Meth. Enzymol. 303, 512–532 (1999).
5. Oh, J. et al. Gene annotation and drug target discovery in Candida albicans with a tagged transposon mutant collection. PLoS Pathog. 6, e1001140 (2010).
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