Author:
Takahashi Eizo,Motooka Daisuke,Nakamura Shota,Miyoshi Shin-ichi,Chowdhury Goutam,Mukhopadhyay Asish K.,Dutta Shanta,Morita Daichi,Iida Tetsuya,Okamoto Keinosuke
Abstract
AbstractWe examined the stools of 23 patients in Kolkata, who were diagnosed as cholera patients becauseVibrio choleraeO1 was detected from their stools by culturing methods, and further explored by metagenomic sequencing analysis. Subsequently, the presence of the gene encoding A subunit of cholera toxin (ctxA) and the cholera toxin (CT) level in these stool samples were examined.ctxAwas examined by both metagenomic sequencing analysis and polymerase chain reaction. In these examinations, two samples did not show positive in any of these tests. The metagenomic analysis showed that the genes forStreptococcus pneumoniaeandSalmonella entericawere present in the stools of these two patients, respectively. Therefore, these two patients were not considered to have diarrhea due toV. choleraeinfection. From these results, we predicted that some Kolkata residents harbor a small number ofV. choleraein their intestines as a form of subclinical infection withV. cholerae. Next, we analyzed the stool samples of 22 diarrhea patients from whichV. choleraewas not isolated. The results showed that 3 of the patients seemed to have subclinical infection ofV. choleraebased on the amount of the genes. These results indicated that subclinical infections withV. choleraeO1 occur in Kolkata.
Funder
Japan Agency for Medical Research and Development
Publisher
Springer Science and Business Media LLC
Reference28 articles.
1. Nair, G. B. et al. Emerging trends in the etiology of enteric pathogens as evidenced from an active surveillance of hospitalized diarrhoeal patients in Kolkata, India. Gut. Pathog. 2, 4–16 (2010).
2. Manna, B. et al. Determinants of health care seeking for diarrheal illness in young children in urban slums of Kolkata, India. Am. J. Trop. Med. Hyg. 89, 56–61 (2013).
3. Laboratory methods for the diagnosis of epidemic dysentery and cholera, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. http://apps.who.int/iris/bitstream/handle/10665/66885/WHO_CDS_CSR_EDC_99.8.pdf;jsessionid=1C73A0E660787FA2302167BF7236A995?sequence=1 (1999).
4. Nelson, E. J., Nelson, D. S., Salam, M. A. & Sack, D. A. Antibiotics for both moderate and severe cholera. N. Engl. J. Med. 364, 5–7 (2011).
5. Harris, J. B., LaRocque, R. C., Qadri, F., Ryan, E. T. & Calderwood, S. B. Cholera. Lancet 379, 2466–2476 (2012).
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献