Author:
Endo Shoichiro,Tada Tatsuya,Oshiro Satoshi,Hishinuma Tomomi,Tohya Mari,Watanabe Shin,Sekiguchi Jun-Ichiro,Abe Masaki,Nakada Koji,Kirikae Teruo
Abstract
AbstractThe emergence and dissemination of carbapenem-resistant species of Acinetobacter and Pseudomonas have become a serious health concern. Routine antimicrobial disk susceptibility tests in clinical laboratories cannot distinguish between isolates that are highly carbapenem-resistant and those that are moderately carbapenem-resistant. The present study describes antimicrobial susceptibility tests using disks containing high doses (1000 μg) of meropenem. The diameters of inhibition zones were significantly negatively correlated with the MICs of Pseudomonas and Acinetobacter species for meropenem (R2: 0.93 and 0.91, respectively) and imipenem (R2: 0.75 and 0.84, respectively). Double disk synergy tests using clavulanic acid or sodium mercaptoacetate can detect ESBL or MBL producers. Susceptibility tests using disks containing high doses of meropenem can easily detect highly carbapenem-resistant isolates in a quantitative manner. These disks may be useful in bacteriological laboratories because of their technical ease, stability, and relatively low cost.
Funder
Japan Society for the Promotion of Science
Institute for Fermentation
Japan Agency for Medical Research and Development
Science and Technology Research Partnership for Sustainable Development
Publisher
Springer Science and Business Media LLC