Author:
Xiang Yong,Li Lizhen,Liu Peng,Yan Ling,Jiang Zeng,Yu Yun,Li Yu,Chen Xiaoyan,Cao Weisheng
Abstract
AbstractAvian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens in China, resulting in great harm to poultry production, and remains widespread in China. Herein, we employed a cross-priming amplification (CPA) approach and a nucleic acid detection device to establish a visual rapid detection method for ALV-J. The sensitivity of CPA, polymerase chain reaction (PCR) and real-time PCR (RT-PCR) was compared, and the three methods were used to detect ALV-J in the cell cultures which inoculated with clinical plasma. The result showed when the amplification reaction was carried out at 60 °C for just 60 min, the sensitivity of CPA was 10 times higher than conventional PCR, with high specificity, which was comparable with RT-PCR, based on detection of 123 cell cultures which inoculated with clinical plasma, the coincidence rate with real-time PCR was 97.3% (71/73). CPA detection of ALV-J does not require an expensive PCR instrument; a simple water bath or incubator is sufficient for complete DNA amplification, and the closed nucleic acid detection device avoids aerosol pollution, making judgment of results more intuitive and objective. The CPA assay would be a promising simple, rapid and sensitive method for identification of ALV-J.
Funder
Key-Area Research and Development Program of Guangdong Province
the China Meat-Type Chicken Research System
the Guangdong Poultry Industry Technology System
Publisher
Springer Science and Business Media LLC
Reference34 articles.
1. King, A. M. Q. et al. Changes to taxonomy and the international code of virus classification and nomenclature ratified by the International Committee on Taxonomy of Viruses (2018). Arch. Virol. 163, 2601–2631 (2018).
2. Bande, F., Arshad, S. S. & Omar, A. R. Isolation and metagenomic identification of avian leukosis virus associated with mortality in broiler chicken. Adv. Virol. 2016, 9058403 (2016).
3. Su, Q. et al. Molecular characteristics of avian leukosis viruses isolated from indigenous chicken breeds in China. Poult. Sci. 97, 2917–2925 (2018).
4. Bova, C. A., Olsen, J. C. & Swanstrom, R. The avian retrovirus env gene family: Molecular analysis of host range and antigenic variants. J. Virol. 62, 75–83 (1988).
5. Smith, E. J., Crittenden, L. B. & Whitson, A. K. Radioimmunoassay for the envelope glycoprotein of subgroup E avian leukosis-sarcoma viruses. Virology 84, 331–340 (1978).
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