Author:
Trusiak Maciej,Cywińska Maria,Micó Vicente,Picazo-Bueno José Ángel,Zuo Chao,Zdańkowski Piotr,Patorski Krzysztof
Abstract
AbstractUtilizing the refractive index as the endogenous contrast agent to noninvasively study transparent cells is a working principle of emerging quantitative phase imaging (QPI). In this contribution, we propose the Variational Hilbert Quantitative Phase Imaging (VHQPI)—end-to-end purely computational add-on module able to improve performance of a QPI-unit without hardware modifications. The VHQPI, deploying unique merger of tailored variational image decomposition and enhanced Hilbert spiral transform, adaptively provides high quality map of sample-induced phase delay, accepting particularly wide range of input single-shot interferograms (from off-axis to quasi on-axis configurations). It especially promotes high space-bandwidth-product QPI configurations alleviating the spectral overlapping problem. The VHQPI is tailored to deal with cumbersome interference patterns related to detailed locally varying biological objects with possibly high dynamic range of phase and relatively low carrier. In post-processing, the slowly varying phase-term associated with the instrumental optical aberrations is eliminated upon variational analysis to further boost the phase-imaging capabilities. The VHQPI is thoroughly studied employing numerical simulations and successfully validated using static and dynamic cells phase-analysis. It compares favorably with other single-shot phase reconstruction techniques based on the Fourier and Hilbert–Huang transforms, both in terms of visual inspection and quantitative evaluation, potentially opening up new possibilities in QPI.
Funder
Narodowe Centrum Nauki
Polish National Agency for Academic Exchange
Ministerio de Economía, Industria y Competitividad, Gobierno de España
National Natural Science Foundation of China
Outstanding Youth Foundation of Jiangsu Province of China
Publisher
Springer Science and Business Media LLC
Reference91 articles.
1. Evanko, D., Heinrichs, A. & Rosenthal, C. Milestones in light microscopy. Nat. Cell Biol. 11, S5–S20 (2009).
2. Park, Y., Depeursinge, C. & Popescu, G. Quantitative phase imaging in biomedicine. Nat. Photon. 12, 578–589 (2018).
3. Popescu, G. Quantitative Phase Imaging of Cells and Tissues (McGraw-Hill, New York, 2011).
4. Shaked, N. T., Zalevsky, Z. & Satterwhite, L. L. Biomedical Optical Phase Microscopy and Nanoscopy (Academic Press, London, 2012).
5. Gabor, D. A new microscopic principle. Nature 161, 777–778 (1948).
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献