Author:
Garrido-Jareño Marta,Puchades-Carrasco Leonor,Orti-Pérez Leticia,Sahuquillo-Arce José Miguel,del Carmen Meyer-García María,Mollar-Maseres Joan,Lloret-Sos Carmina,Gil-Brusola Ana,López-Hontangas José Luis,Beltrán-Garrido José Manuel,Pemán-García Javier,Pineda-Lucena Antonio
Abstract
AbstractIncidence of pneumococcal disease has increased worldwide in recent years. Response to pneumococcal vaccine is usually measured using the multiserotype enzyme-linked immunosorbent assay (ELISA) pneumococcal test. However, this approach presents several limitations. Therefore, the introduction of new and more robust analytical approaches able to provide information on the efficacy of the pneumococcal vaccine would be very beneficial for the clinical management of patients. Surface plasmon resonance (SPR) has been shown to offer a valuable understanding of vaccines’ properties over the last years. The aim of this study is to evaluate the reliability of SPR for the anti-pneumococcal capsular polysaccharides (anti-PnPs) IgGs quantification in vaccinated. Fast protein liquid chromatography (FPLC) was used for the isolation of total IgGs from serum samples of vaccinated patients. Binding-SPR assays were performed to study the interaction between anti-PnPs IgGs and PCV13. A robust correlation was found between serum levels of anti-PnPs IgGs, measured by ELISA, and the SPR signal. Moreover, it was possible to correctly classify patients into “non-responder”, “responder” and “high-responder” groups according to their specific SPR PCV13 response profiles. SPR technology provides a valuable tool for reliably characterize the interaction between anti-PnPs IgGs and PCV13 in a very short experimental time.
Funder
Health Research Institute La Fe
Conselleria de Sanitat Universal i Salut Pública
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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