Author:
Matthies Doreen,Lee Nathanael Y. J.,Gatera Ian,Pasolli H. Amalia,Zhao Xiaowei,Liu Hui,Walpita Deepika,Liu Zhe,Yu Zhiheng,Ioannou Maria S.
Abstract
AbstractExtracellular vesicles (EVs) are important mediators of cell-to-cell communication and have been implicated in several pathologies including those of the central nervous system. They are released by all cell types, including neurons, and are highly heterogenous in size and composition. Yet much remains unknown regarding the biophysical characteristics of different EVs. Here, using cryo-electron microscopy (cryoEM), we analyzed the size distribution and morphology of EVs released from primary cortical neurons. We discovered massive macromolecular clusters on the luminal face of EV membranes. These clusters are predominantly found on medium-sized vesicles, suggesting that they may be specific to microvesicles as opposed to exosomes. We propose that these clusters serve as microdomains for EV signaling and play an important role in EV physiology.
Funder
Howard Hughes Medical Institute
Publisher
Springer Science and Business Media LLC
Cited by
17 articles.
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