Author:
Barabona Godfrey,Ngare Isaac,Kamori Doreen,Nkinda Lilian,Kosugi Yusuke,Mawazo Ambele,Ekwabi Rayi,Kinasa Gloria,Chuwa Harrison, ,Matsuno Keita,Nao Naganori,Sawa Hirofumi,Tanaka Shinya,Tsuda Masumi,Wang Lei,Oda Yoshikata,Ferdous Zannatul,Shishido Kenji,Fukuhara Takasuke,Tamura Tomokazu,Suzuki Rigel,Suzuki Saori,Ito Hayato,Kaku Yu,Misawa Naoko,Plianchaisuk Arnon,Guo Ziyi,Hinay Alfredo A.,Uriu Keiya,Tolentino Jarel Elgin M.,Chen Luo,Pan Lin,Suganami Mai,Chiba Mika,Yoshimura Ryo,Yasuda Kyoko,Iida Keiko,Ohsumi Naomi,Strange Adam P.,Tanaka Shiho,Yoshimura Kazuhisa,Sadamasu Kenji,Nagashima Mami,Asakura Hiroyuki,Yoshida Isao,Nakagawa So,Shirakawa Kotaro,Takaori-Kondo Akifumi,Nagata Kayoko,Nomura Ryosuke,Horisawa Yoshihito,Tashiro Yusuke,Kawai Yugo,Takayama Kazuo,Hashimoto Rina,Deguchi Sayaka,Watanabe Yukio,Sakamoto Ayaka,Yasuhara Naoko,Hashiguchi Takao,Suzuki Tateki,Kimura Kanako,Sasaki Jiei,Nakajima Yukari,Yajima Hisano,Irie Takashi,Kawabata Ryoko,Tabata Kaori,Ikeda Terumasa,Nasser Hesham,Shimizu Ryo,Begum M. S. T. Monira,Jonathan Michael,Mugita Yuka,Takahashi Otowa,Ichihara Kimiko,Motozono Chihiro,Ueno Takamasa,Toyoda Mako,Saito Akatsuki,Shofa Maya,Shibatani Yuki,Nishiuchi Tomoko,Sato Kei,Sunguya Bruno,Ueno Takamasa
Abstract
AbstractThe ongoing vaccination efforts and exposure to endemic and emerging coronaviruses can shape the population's immunity against this group of viruses. In this study, we investigated neutralizing immunity against endemic and emerging coronaviruses in 200 Tanzanian frontline healthcare workers (HCWs). Despite low vaccination rates (19.5%), we found a high SARS-CoV-2 seroprevalence (94.0%), indicating high exposure in these HCWs. Next, we determined the neutralization capacity of antisera against human coronavirus NL63, and 229E, SARS-CoV-1, MERS-CoV and SARS-CoV-2 (including Omicron subvariants: BA.1, BQ.1.1 and XBB.1.5) using pseudovirus neutralization assay. We observed a broad range of neutralizing activity in HCWs, but no neutralization activity detected against MERS-CoV. We also observed a strong correlation between neutralizing antibody titers for SARS-CoV-2 and SARS-CoV-1, but not between other coronaviruses. Cross-neutralization titers against the newer Omicron subvariants, BQ.1.1 and XBB.1.5, was significantly reduced compared to BA.1 and BA.2 subvariants. On the other hand, the exposed vaccinated HCWs showed relatively higher median cross-neutralization titers against both the newer Omicron subvariants and SARS-CoV-1, but did not reach statistical significance. In summary, our findings suggest a broad range of neutralizing potency against coronaviruses in Tanzanian HCWs with detectable neutralizing immunity against SARS-CoV-1 resulting from SARS-CoV-2 exposure.
Funder
Japan Society for the Promotion of Science
Terumo Foundation for Life Sciences and Arts
Publisher
Springer Science and Business Media LLC