Author:
Jeong Younsik,Choi Woon-Yong,Park Areumi,Lee Yeon-Ji,Lee Youngdeuk,Park Gun-Hoo,Lee Su-Jin,Lee Won-Kyu,Ryu Yong-Kyun,Kang Do-Hyung
Abstract
AbstractSerum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50–70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.
Funder
Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science and ICT
Publisher
Springer Science and Business Media LLC
Reference70 articles.
1. Maurer, H. R. Towards chemically-defined, serum-free media for mammalian cell culture. In: Animal Cell Culture a practical approach. (ed Freshney, R. I.) 13–31 (IRL Press, Oxford, 1986).
2. Klein, R. & Dumble, L. Transmission of Creutzfeldt-Jakob disease by blood transfusion. The Lancet 341, 768 (1993).
3. Jochems, C. E., Van Der Valk, J. B., Stafleu, F. R. & Baumans, V. The use of fetal bovine serum: ethical or scientific problem?. ATLA-NOTTINGHAM 30, 219–228 (2002).
4. Eloit, M. Risks of virus transmission associated with animal sera or substitutes and methods of control. Dev. Biol. Stand. 99, 9–16 (1999).
5. Shah, G. Why do we still use serum in the production of biopharmaceuticals?. Dev. Biol. Stand. 99, 17–22 (1999).
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献