Author:
Davison Jack R.,Rajwani Rahim,Zhao Gengxiang,Bewley Carole A.
Abstract
AbstractChrysophaeum taylorii is a member of an understudied clade of marine algae that can be responsible for harmful coastal blooms and is known to accumulate bioactive natural products including antibiotics of the chrysophaentin class. Whole genome sequencing of laboratory-cultivated samples revealed an extensive and diverse complement of secondary metabolite biosynthetic genes in C. taylorii, alongside a small microbiome with a more limited biosynthetic potential. 16S microbiome analysis of laboratory cultured alongside wild-collected samples revealed several common taxa; however, analysis of biosynthetic genes suggested an algal origin for the chrysophaentins, possibly via one of several non-canonical polyketide synthase genes encoded within the genome.
Funder
Division of Intramural Research, National Institute of Diabetes and Digestive and Kidney Diseases, United States
Publisher
Springer Science and Business Media LLC
Reference48 articles.
1. Keffer, J. L. et al. Geographic variability and anti-Staphylococcal activity of the chrysophaentins and their synthetic fragments. Mar. Drugs 10, 1103–1125 (2012).
2. Lobban, C. S. & Tsuda, R. T. Revised checklist of benthic marine macroalgae and seagrasses of Guam and Micronesia. Micronesica 35–36, 54–99 (2003).
3. Caronni, S., Delaria, M. A. & Macri, G. F. B. A pilot study on the effects of temperature on the blooms of the benthic mucilage-forming microalga Chrysophaeum taylorii. J. Environ. Sci. Eng. B 4, 9–16 (2015).
4. Davison, J. R. & Bewley, C. A. Antimicrobial chrysophaentin analogs identified from laboratory cultures of the marine microalga Chrysophaeum taylorii. J. Nat. Prod. 82, 148–153 (2019).
5. Wetherbee, R. et al. New pelagophytes show a novel mode of algal colony development and reveal a perforated theca that may define the class. J. Phycology 57, 396–411 (2020).