Author:
Sugiura Takanori,Hashimoto Kazuhiko,Kikuta Kazutaka,Anazawa Ukei,Nomura Takeshi,Kameyama Akihiko
Abstract
AbstractMucoepidermoid carcinoma (MEC) is the most frequent of the rare salivary gland malignancies. We previously reported high expression of Mucin 1 (MUC1) modified with sialylated core-2 O-glycans in MEC by using tissue homogenates. In this study, we characterised glycan structures of MEC and identified the localisation of cells expressing these distinctive glycans on MUC1. Mucins were extracted from the frozen tissues of three patients with MEC, and normal salivary glands (NSGs) extracted from seven patients, separated by supported molecular matrix electrophoresis (SMME) and the membranes stained with various lectins. In addition, formalin-fixed, paraffin-embedded sections from three patients with MEC were subjected to immunohistochemistry (IHC) with various monoclonal antibodies and analysed for C2GnT-1 expression by in situ hybridisation (ISH). Lectin blotting of the SMME membranes revealed that glycans on MUC1 from MEC samples contained α2,3-linked sialic acid. In IHC, MUC1 was diffusely detected at MEC-affected regions but was specifically detected at apical membranes in NSGs. ISH showed that C2GnT-1 was expressed at the MUC1-positive in MEC-affected regions but not in the NSG. MEC cells produced MUC1 modified with α2,3-linked sialic acid-containing core-2 O-glycans. MUC1 containing these glycans deserves further study as a new potential diagnostic marker of MEC.
Funder
Japan Society for the Promotion of Science
Mizutani Foundation for Glycoscience
Publisher
Springer Science and Business Media LLC
Reference30 articles.
1. Suhela, R. & Gattu, V. Study of morphological subtypes of major salivary gland tumors. Perspect. Med. Res. 3, 24–28 (2017).
2. Naggar, A. K. et al. WHO Classification of Head and Neck Tumors 4th edn, 159–164 (World Health Organization, 2017).
3. Janet-Ofelia, G. C. et al. Mucoepidermoid carcinoma of the salivary glands: Survival and prognostic factors. J. Maxillofac. Oral. Surg. 16, 431–437 (2017).
4. Robinson, L., van Heerden, M. B., Ker-Fox, J. G., Hunter, K. D. & van Heerdenet, W. F. Expression of mucins in salivary gland mucoepidermoid carcinoma. Head Neck Pathol. 15, 491–502 (2021).
5. Alos, L. et al. Expression of membrane-bound mucins (MUC1 and MUC4) and secreted mucins (MUC2, MUC5AC, MUC5B, MUC6 and MUC7) in mucoepidermoid carcinomas of salivary glands. Am. J. Surg. Pathol. 29, 806–813 (2005).
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