Abstract
AbstractIron is a limiting factor in such a condition that usually is sequestered by the host during polymicrobial infections of Pseudomonas aeruginosa and Staphylococcus aureus. This study aimed to investigate the interaction of S. aureus and P. aeruginosa, which alters iron-related sigma factors regulation and antibiotic resistance. The antibiotic resistance of P. aeruginosa and S. aureus was investigated in a L929 cell culture model. The expression level of pvdS, hasI (P. aeruginosa sigma factors), and sigS (S. aureus sigma factor) genes was determined using Quantitative Real-Time PCR. pvdS and hasI were downregulated during co-culture with S. aureus, while the susceptibility to carbapenems increased (p-value < 0.0001). Also, there was a direct significant relationship between resistance to vancomycin with sigS. Regarding the findings of the current study, iron-related sigma factors of P. aeruginosa and S. aureus play a role in induction susceptibility to various antibiotics, including carbapenems and vancomycin.
Funder
This study was supported by Hamadan University of Medical Sciences
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Tahmasebi, H., Maleki, F., Dehbashi, S. & Arabestani, M. R. Role and function of KPC and MBL enzymes in increasing the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds. J. Babol Univ. Med. Sci. 21, 127–134 (2019).
2. Barnabie, P. M. & Whiteley, M. Iron-mediated control of Pseudomonas aeruginosa–Staphylococcus aureus interactions in the cystic fibrosis lung. J. Bacteriol. 197, 2250–2251. https://doi.org/10.1128/jb.00303-15 (2015).
3. Bjarnsholt, T. The role of bacterial biofilms in chronic infections. APMIS. Suppl. https://doi.org/10.1111/apm.12099 (2013).
4. Gaines, J. M. et al. Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen. Microbiology 153, 4219–4233. https://doi.org/10.1099/mic.0.2007/011338-0 (2007).
5. Nguyen, A. T. et al. Adaptation of iron homeostasis pathways by a Pseudomonas aeruginosa pyoverdine mutant in the cystic fibrosis lung. J. Bacteriol. 196, 2265–2276. https://doi.org/10.1128/JB.01491-14 (2014).
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