Author:
Tekle Yonas I.,Wang Fang,Tran Hanh,Hayes T. Danielle,Ryan Joseph F.
Abstract
AbstractTo date, genomic analyses in amoebozoans have been mostly limited to model organisms or medically important lineages. Consequently, the vast diversity of Amoebozoa genomes remain unexplored. A draft genome of Cochliopodium minus, an amoeba characterized by extensive cellular and nuclear fusions, is presented. C. minus has been a subject of recent investigation for its unusual sexual behavior. Cochliopodium’s sexual activity occurs during vegetative stage making it an ideal model for studying sexual development, which is sorely lacking in the group. Here we generate a C. minus draft genome assembly. From this genome, we detect a substantial number of lateral gene transfer (LGT) instances from bacteria (15%), archaea (0.9%) and viruses (0.7%) the majority of which are detected in our transcriptome data. We identify the complete meiosis toolkit genes in the C. minus genome, as well as the absence of several key genes involved in plasmogamy and karyogamy. Comparative genomics of amoebozoans reveals variation in sexual mechanism exist in the group. Similar to complex eukaryotes, C. minus (some amoebae) possesses Tyrosine kinases and duplicate copies of SPO11. We report a first example of alternative splicing in a key meiosis gene and draw important insights on molecular mechanism of sex in C. minus using genomic and transcriptomic data.
Funder
National Science Foundation
Foundation for the National Institutes of Health
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. Adl, S. M. et al. Revisions to the classification, nomenclature, and diversity of eukaryotes. J. Eukaryot. Microbiol. 66, 4–119. https://doi.org/10.1111/jeu.12691 (2019).
2. Anderson, O. R. The role of bacterial-based protist communities in aquatic and soil ecosystems and the carbon biogeochemical cycle, with emphasis on naked amoebae. Acta Protozool. 51, 209–221 (2012).
3. Declerck, P., Behets, J., van Hoef, V. & Ollevier, F. Detection of Legionella spp. and some of their amoeba hosts in floating biofilms from anthropogenic and natural aquatic environments. Water Res. 41, 3159–3167. https://doi.org/10.1016/j.watres.2007.04.011 (2007).
4. Huws, S. A., Smith, A. W., Enright, M. C., Wood, P. J. & Brown, M. R. Amoebae promote persistence of epidemic strains of MRSA. Environ. Microbiol. 8, 1130–1133. https://doi.org/10.1111/j.1462-2920.2006.00991.x (2006).
5. Bonner, J. T. A descriptive study of the development of the slime mold Dictyostelium discoideum. Am. J. Bot. 31, 175–182 (1944).
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