Author:
Kayman Kürekçi Gülsüm,Kural Mangit Ecem,Koyunlar Cansu,Unsal Seyda,Saglam Berk,Ergin Bora,Gizer Merve,Uyanik Ismail,Boustanabadimaralan Düz Niloufar,Korkusuz Petek,Talim Beril,Purali Nuhan,Hughes Simon M.,Dincer Pervin R.
Abstract
AbstractDesmin is a muscle-specific intermediate filament protein that has fundamental role in muscle structure and force transmission. Whereas human desmin protein is encoded by a single gene, two desmin paralogs (desma and desmb) exist in zebrafish. Desma and desmb show differential spatiotemporal expression during zebrafish embryonic and larval development, being similarly expressed in skeletal muscle until hatching, after which expression of desmb shifts to gut smooth muscle. We generated knockout (KO) mutant lines carrying loss-of-function mutations for each gene by using CRISPR/Cas9. Mutants are viable and fertile, and lack obvious skeletal muscle, heart or intestinal defects. In contrast to morphants, knockout of each gene did not cause any overt muscular phenotype, but did alter calcium flux in myofibres. These results point to a possible compensation mechanism in these mutant lines generated by targeting nonsense mutations to the first coding exon.
Funder
Medical Research Council
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Publisher
Springer Science and Business Media LLC
Cited by
15 articles.
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