Author:
Tirloni Lucas,Braz Gloria,Nunes Rodrigo Dutra,Gandara Ana Caroline Paiva,Vieira Larissa Rezende,Assumpcao Teresa Cristina,Sabadin Gabriela Alves,da Silva Renato Martins,Guizzo Melina Garcia,Machado Josias Alves,Costa Evenilton Pessoa,Santos Daniele,Gomes Helga Fernandes,Moraes Jorge,dos Santos Mota Maria Beatriz,Mesquita Rafael Dias,de Souza Leite Milane,Alvarenga Patricia Hessab,Lara Flavio Alves,Seixas Adriana,da Fonseca Rodrigo Nunes,Fogaça Andrea C.,Logullo Carlos,Tanaka Aparecida Sadae,Daffre Sirlei,Oliveira Pedro L.,da Silva Vaz Itabajara,Ribeiro José M. C.
Abstract
AbstractTo further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species.
Funder
Division of Intramural Research, National Institute of Allergy and Infectious Diseases
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Code 001
Publisher
Springer Science and Business Media LLC
Cited by
23 articles.
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