Author:
Loutfi Hadi,Pellen Fabrice,Le Jeune Bernard,Lteif Roger,Kallassy Mireille,Le Brun Guy,Abboud Marie
Abstract
AbstractIn microbiology, monitoring the growth of any microorganism in culture is important for studying and optimizing the growth kinetics, the biomass and the metabolite production. In this work, we show that laser speckle imaging is a reliable technique that can be used to perform real-time monitoring of bacteria growth kinetic in liquid culture media. Speckle parameters, specifically speckle grain size and the spatial contrast of the speckle images, and standard analytical parameters (optical density, pH and colony forming units) were measured during the culture of different strains of Bacillus thuringiensis. Our results show that both speckle grain size and spatial contrast decrease with bacterial growth. Furthermore, speckle parameters are sensitive to the fermentation conditions. Statistical analysis revealed a relatively high correlation between speckle and analytical parameters.
Funder
Seventh Framework Programme
Conseil National de la Recherche Scientifique
Saint Joseph University
Publisher
Springer Science and Business Media LLC
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