Author:
Jang Hye Ryoun,Kim Minjung,Hong Sungjun,Lee Kyungho,Park Mee Yeon,Yang Kyeong Eun,Lee Cheol-Jung,Jeon Junseok,Lee Kyo Won,Lee Jung Eun,Park Jae Berm,Kim Kyunga,Kwon Ghee Young,Kim Yoon Goo,Kim Dae Joong,Huh Wooseong
Abstract
AbstractWe investigated the clinical relevance of urinary cytokines/chemokines reflecting intrarenal immunologic micromilieu as prognostic markers and the optimal measurement timing after living donor kidney transplantation (LDKT). This prospective cohort study included 77 LDKT patients who were followed for ≥ 5 years. Patients were divided into control (n = 42) or acute rejection (AR, n = 35) group. Early AR was defined as AR occurring within 3 months. Serum and urine cytokines/chemokines were measured serially as follows: intraoperative, 8/24/72 h, 1 week, 3 months, and 1 year after LDKT. Intrarenal total leukocytes, T cells, and B cells were analyzed with immunohistochemistry followed by tissueFAXS. Urinary MCP-1 and fractalkine were also analyzed in a validation cohort. Urinary MCP-1 after one week was higher in the AR group. Urinary MCP-1, fractalkine, TNF-α, RANTES, and IL-6 after one week were significantly higher in the early AR group. Intrarenal total leukocytes and T cells were elevated in the AR group compared with the control group. Urinary fractalkine, MCP-1, and IL-10 showed positive correlation with intrarenal leukocyte infiltration. Post-KT 1 week urinary MCP-1 showed predictive value in the validation cohort. One-week post-KT urinary MCP-1 may be used as a noninvasive diagnostic marker for predicting AR after LDKT.
Funder
Korea Health Technology R&D Project through the Korea Health Industry Development Institute
Samsung Biomedical Research Institute
National Research Foundation of Republic of Korea
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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