Author:
Baison J.,Zhou Linghua,Forsberg Nils,Mörling Tommy,Grahn Thomas,Olsson Lars,Karlsson Bo,Wu Harry X.,Mellerowicz Ewa J.,Lundqvist Sven-Olof,García-Gil María Rosario
Abstract
AbstractThrough the use of genome-wide association studies (GWAS) mapping it is possible to establish the genetic basis of phenotypic trait variation. Our GWAS study presents the first such effort in Norway spruce (Picea abies (L). Karst.) for the traits related to wood tracheid characteristics. The study employed an exome capture genotyping approach that generated 178 101 Single Nucleotide Polymorphisms (SNPs) from 40 018 probes within a population of 517 Norway spruce mother trees. We applied a least absolute shrinkage and selection operator (LASSO) based association mapping method using a functional multi-locus mapping approach, with a stability selection probability method as the hypothesis testing approach to determine significant Quantitative Trait Loci (QTLs). The analysis has provided 30 significant associations, the majority of which show specific expression in wood-forming tissues or high ubiquitous expression, potentially controlling tracheids dimensions, their cell wall thickness and microfibril angle. Among the most promising candidates based on our results and prior information for other species are: Picea abies BIG GRAIN 2 (PabBG2) with a predicted function in auxin transport and sensitivity, and MA_373300g0010 encoding a protein similar to wall-associated receptor kinases, which were both associated with cell wall thickness. The results demonstrate feasibility of GWAS to identify novel candidate genes controlling industrially-relevant tracheid traits in Norway spruce.
Funder
Bio4Energy
Knut and Alice Wallenberg Foundation
Kemp Foundation
Swedish University of Agricultural Sciences
Publisher
Springer Science and Business Media LLC
Reference115 articles.
1. Mäkinen, H., Saranpää, P. & Linder, S. Effect of Growth rate on fibre characteristics in Norway Spruce (Picea abies (L.) Karst.). (2002).
2. Lundqvist, S.-O. & Gardiner, B. Key products of the forest-based industries and their demands on wood raw material properties. Joensuu, Finland, https://www.efi.int/files/attachments/publications/eforwood/efi_tr_71.pdf, [March 23, 2013] (2011).
3. Brändström, J. Morphology of Norway spruce tracheids with emphasis on cell wall organisation. vol. 237 (2002).
4. Dutilleul, P., Herman, M. & Avella-Shaw, T. Growth rate effects on correlations among ring width, wood density, and mean tracheid length in Norway spruce (Picea abies). Can. J. For. Res. 28, 56–68 (1998).
5. Hannrup, B. & Ekberg, I. Age-age correlations for tracheid length and wood density in Pinus sylvestris. Can. J. For. Res. 28, 1373–1379 (1998).
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