Author:
Ölander Christine,Edvardsson Rasmussen Jesper,Eriksson Per Olof,Laurell Göran,Rask-Andersen Helge,Bergquist Jonas
Abstract
AbstractThe endolymphatic sac (ES) is the third part of the inner ear, along with the cochlea and vestibular apparatus. A refined sampling technique was developed to analyse the proteomics of ES endolymph. With a tailored solid phase micro-extraction probe, five ES endolymph samples were collected, and six sac tissue biopsies were obtained in patients undergoing trans-labyrinthine surgery for sporadic vestibular schwannoma. The samples were analysed using nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) to identify the total number of proteins. Pathway identification regarding molecular function and protein class was presented. A total of 1656 non-redundant proteins were identified, with 1211 proteins detected in the ES endolymph. A total of 110 proteins were unique to the ES endolymph. The results from the study both validate a strategy for in vivo and in situ human sampling during surgery and may also form a platform for further investigations to better understand the function of this intriguing part of the inner ear.
Funder
Foundation Tysta Skolan
Uppsala University Hospital ALF Grants
Foundation Acta Otolaryngologica
the Swedish Research Council
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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