Author:
Rybicka Magda,Czaplewska Paulina,Rzymowska Jolanta,Sofińska-Chmiel Weronika,Wójcik-Mieszawska Sylwia,Lewtak Kinga,Węgrzyn Katarzyna,Jurczak Przemysław,Szpiech Agata,Nowak Jakub,Musiał Natalia,Fiołka Marta J.
Abstract
AbstractThe present research shows the antitumor activity of a protein-polysaccharide complex Venetin-1 obtained from the coelomic fluid of Dendrobaena veneta earthworms against A549 cancer cells. The investigations are a continuation of experiments on the antitumor activity of coelomic fluid obtained from this species. The Venetin-1 nanoparticle was obtained after thermal treatment of the coelomic fluid, separation from coelomocytes, filtration, and lyophilization. The preparation showed a selective effect on cancer cells, whereas normal cells were unaffected. Venetin-1 was effective against the lung cancer cells at doses of 31.3 and 62.5 µg/ml, and the results were imaged using light microscopy and scanning electron microscopy (SEM). The cells died mainly via the apoptosis pathway. Necrotic cells appeared sporadically in the microscopic view. SEM imaging revealed complete destruction of the A549 cells after the incubation with Venetin-1. The atomic force microscopy (AFM) analyses showed changes in the topography, peak force error images, and Young’s modulus (elasticity) of the A549 cells after the incubation with Venetin-1. The transmission electron cryomicroscopy (Cryo-TEM) analysis indicated a polymeric nature of the analyzed preparation. The samples of Venetin-1 showed a very homogeneous size profile with the microparticle size of approximately 58.23 nm. A significant decrease in Venetin-1 binding to sphingomyelin was observed. Venetin-1 lost its pore-forming activity or deactivation of the pore-forming activity occurred. This confirms the absence of hemolytic capacity of Venetin-1 towards red blood cells. The conducted analyses show the suitability of the obtained complex for biomedical research. The next step will consist in analyses of the effect of Venetin-1 on the immune system in mice.
Publisher
Springer Science and Business Media LLC
Reference68 articles.
1. Bade, B. C. & Dela, C. C. S. Lung cancer 2020: Epidemiology, etiology, and prevention. Clin. Chest Med. 41. https://doi.org/10.1016/j.ccm.2019.10.001 (2020).
2. Herbst, R., Morgensztern, D. & Boshoff, C. The biology and management of non-small cell lung cancer. Nature 553, 446–454 (2018).
3. Hecht, S. S. Cigarette smoking and lung cancer: Chemical mechanisms and approaches to prevention. Lancet Oncol. 8, 461–469 (2002).
4. Akopyan, G. & Bonavida, B. Understanding tobacco smoke carcinogen NNK and lung tumorigenesis (Review). Int. J. Oncol. 29, 745–752 (2006).
5. Barta, J. A., Powell, C. A. & Wisnivesky, J. P. Global epidemiology of lung cancer. Ann. Glob. Health https://doi.org/10.5334/aogh.2419 (2019).
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献