Author:
Sun Yan,Yu Qichao,Li Lei,Mei Zhanlong,Zhou Biaofeng,Liu Shang,Pan Taotao,Wu Liang,Lei Ying,Liu Longqi,Drmanac Radoje,Ma Kun,Liu Shiping
Abstract
Abstract
Recent studies show that non-coding RNAs (ncRNAs) can regulate the expression of protein-coding genes and play important roles in mammalian development. Previous studies have revealed that during C. elegans (Caenorhabditis elegans) embryo development, numerous genes in each cell are spatiotemporally regulated, causing the cell to differentiate into distinct cell types and tissues. We ask whether ncRNAs participate in the spatiotemporal regulation of genes in different types of cells and tissues during the embryogenesis of C. elegans. Here, by using marker-free full-length high-depth single-cell RNA sequencing (scRNA-seq) technique, we sequence the whole transcriptomes from 1031 embryonic cells of C. elegans and detect 20,431 protein-coding genes, including 22 cell-type-specific protein-coding markers, and 9843 ncRNAs including 11 cell-type-specific ncRNA markers. We induce a ncRNAs-based clustering strategy as a complementary strategy to the protein-coding gene-based clustering strategy for single-cell classification. We identify 94 ncRNAs that have never been reported to regulate gene expressions, are co-expressed with 1208 protein-coding genes in cell type specific and/or embryo time specific manners. Our findings suggest that these ncRNAs could potentially influence the spatiotemporal expression of the corresponding genes during the embryogenesis of C. elegans.
Funder
Natural Science Foundation of Guangdong Province, China
Shenzhen Key Laboratory of Single-Cell Omics
the Shenzhen Municipal Government of China Peacock Plan
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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