Author:
Tsugawa Satoru,Yamasaki Yuki,Horiguchi Shota,Zhang Tianhao,Muto Takara,Nakaso Yosuke,Ito Kenshiro,Takebayashi Ryu,Okano Kazunori,Akita Eri,Yasukuni Ryohei,Demura Taku,Mimura Tetsuro,Kawaguchi Ken’ichi,Hosokawa Yoichiroh
Abstract
AbstractThe stiffness of a plant cell in response to an applied force is determined not only by the elasticity of the cell wall but also by turgor pressure and cell geometry, which affect the tension of the cell wall. Although stiffness has been investigated using atomic force microscopy (AFM) and Young’s modulus of the cell wall has occasionally been estimated using the contact-stress theory (Hertz theory), the existence of tension has made the study of stiffness more complex. Elastic shell theory has been proposed as an alternative method; however, the estimation of elasticity remains ambiguous. Here, we used finite element method simulations to verify the formula of the elastic shell theory for onion (Allium cepa) cells. We applied the formula and simulations to successfully quantify the turgor pressure and elasticity of a cell in the plane direction using the cell curvature and apparent stiffness measured by AFM. We conclude that tension resulting from turgor pressure regulates cell stiffness, which can be modified by a slight adjustment of turgor pressure in the order of 0.1 MPa. This theoretical analysis reveals a path for understanding forces inherent in plant cells.
Funder
MEXT KAKENHI
Japan Science and Technology Agency
Publisher
Springer Science and Business Media LLC
Cited by
15 articles.
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