Using an AraC-based three-hybrid system to detect biocatalysts in vivo-Reference-Cited by-同舟云学术

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Using an AraC-based three-hybrid system to detect biocatalysts in vivo

Published:2000-05 Issue:5 Volume:18 Page:544-547
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Firestine Steven M,Salinas Frank,Nixon Andrew E,Baker Stephen J,Benkovic Stephen J

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://www.nature.com/articles/nbt0500_544.pdf

Reference17 articles.

1. Stemmer, W.P.C. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA 91, 10747–10751 (1994).

2. Zhao, H. et al. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat. Biotechnol. 16, 258–261 (1998).

3. Shao, Z., Zhao, H., Giver, L. & Arnold, F.H. Random-priming in vitro recombination: an effective tool for directed evolution. Nucleic Acid Res. 26, 681–683 (1998).

4. Ostermeier, M., Nixon, A.E., Shim, J.H. & Benkovic, S.J. Combinatorial protein engineering by incremental truncation. Proc. Natl. Acad. Sci. USA 96, 3562–3567 (1999).

5. Nixon, A.E., Firestine, S.M., Salinas, F.G. & Benkovic, S.J. Rational design of a scytalone dehydratase-like enzyme using a structurally homologous protein scaffold. Proc. Natl. Acad. Sci. USA 96, 3568–3571 (1999).

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