Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep21451.pdf
Reference35 articles.
1. Li, J. F. et al. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol 31, 688–691 (2013).
2. Mao, Y. et al. Application of the CRISPR-Cas system for efficient genome engineering in plants. Mol Plant 6, 2008–2011 (2013).
3. Xie, K. & Yang, Y. RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant 6, 1975–1983 (2013).
4. Xie, K., Minkenberg, B. & Yang, Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci USA 112, 3570–3575 (2015).
5. Fauser, F., Schiml, S. & Puchta, H. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J 79, 348–359 (2014).
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