Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep17488.pdf
Reference55 articles.
1. Simmons, L. C. et al. Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies. J Immunol Methods 263, 133–147 (2002).
2. Chen, C. et al. High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain. Biotechnol Bioeng 85, 463–474 (2004).
3. Makino, T., Skretas, G., Kang, T. H. & Georgiou, G. Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies. Metab. Eng. 13, 241–251 (2011).
4. Bothmann, H. & Plückthun, A. The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines. J Biol Chem 275, 17100–17105 (2000).
5. Demarest, S. J. et al. Engineering stability into Escherichia coli secreted Fabs leads to increased functional expression. Protein Eng Des Sel 19, 325–336 (2006).
Cited by 22 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Approaches for high-throughput quantification of periplasmic recombinant proteins;New Biotechnology;2023-11
2. Application of germline antibody features to vaccine development, antibody discovery, antibody optimization and disease diagnosis;Biotechnology Advances;2023-07
3. Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties;mAbs;2023-01-11
4. Engineered Human Antibody with Improved Endothelin Receptor Type A Binding Affinity, Developability, and Serum Persistence Exhibits Excellent Antitumor Potency;Molecular Pharmaceutics;2022-12-23
5. Full-length recombinant antibodies fromEscherichia coli: production, characterization, effector function (Fc) engineering, and clinical evaluation;mAbs;2022-08-26
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3