Multiphoton single-molecule localization by sequential excitation with light minima-Reference-Cited by-同舟云学术

Multiphoton single-molecule localization by sequential excitation with light minima

Published:2022-03-25 Issue:1 Volume:11 Page:
ISSN:2047-7538
Container-title:Light: Science & Applications
language:en
Short-container-title:Light Sci Appl

Author:

Masullo Luciano A.,Stefani Fernando D.^ORCID

Abstract

AbstractUsing sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

Funder

Ministry of Science, Technology and Productive Innovation, Argentina | Agencia Nacional de Promoción Científica y Tecnológica

Publisher

Springer Science and Business Media LLC

Subject

Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials

Link

https://www.nature.com/articles/s41377-022-00763-2.pdf

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