Abstract
AbstractLight serves as the energy source for plants as well as a signal for growth and development during their whole life cycle. Seedling de-etiolation is the most dramatic manifestation of light-regulated plant development processes, as massive reprogramming of the plant transcriptome occurs at this time. Although several studies have reported about organ-specific development and expression induced by light, a systematic analysis of cell-type-specific differentiation and the associated transcriptional regulation is still lacking. Here we obtained single-cell transcriptional atlases for etiolated, de-etiolating and light-grown Arabidopsisthaliana seedlings. Informative cells from shoot and root tissues were grouped into 48 different cell clusters and finely annotated using multiple markers. With the determination of comprehensive developmental trajectories, we demonstrate light modulation of cell fate determination during guard cell specialization and vasculature development. Comparison of expression atlases between wild type and the pifq mutant indicates that phytochrome-interacting factors (PIFs) are involved in distinct developmental processes in endodermal and stomatal lineage cells via controlling cell-type-specific expression of target genes. These results provide information concerning the light signalling networks at the cell-type resolution, improving our understanding of how light regulates plant development at the cell-type and genome-wide levels. The obtained information could serve as a valuable resource for comprehensively investigating the molecular mechanism of cell development and differentiation in response to light.
Publisher
Springer Science and Business Media LLC
Reference77 articles.
1. Von Arnim, A. & Deng, X. W. Light control of seedling development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 215–243 (1996).
2. Chory, J., Peto, C., Feinbaum, R., Pratt, L. & Ausubel, F. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell 58, 991–999 (1989).
3. Neff, M. M. & Van Volkenburgh, E. Light-stimulated cotyledon expansion in Arabidopsis seedlings (The role of phytochrome B). Plant Physiol. 104, 1027–1032 (1994).
4. Wei, H., Kong, D., Yang, J. & Wang, H. Light regulation of stomatal development and patterning: shifting the paradigm from Arabidopsis to grasses. Plant Commun. 1, 100030 (2020).
5. Wang, S. et al. Light regulates stomatal development by modulating paracrine signaling from inner tissues. Nat. Commun. 12, 3403 (2021).
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献