Despite mutation acquisition in hematopoietic stem cells, JMML-propagating cells are not always restricted to this compartment

Author:

Caye Aurélie,Rouault-Pierre KevinORCID,Strullu Marion,Lainey Elodie,Abarrategi Ander,Fenneteau Odile,Arfeuille Chloé,Osman Jennifer,Cassinat Bruno,Pereira Sabrina,Anjos-Afonso Fernando,Currie Erin,Ariza-McNaughton Linda,Barlogis Vincent,Dalle Jean-Hugues,Baruchel André,Chomienne Christine,Cavé Hélène,Bonnet DominiqueORCID

Abstract

Abstract Juvenile myelomonocytic leukemia (JMML) is a rare aggressive myelodysplastic/myeloproliferative neoplasm of early childhood, initiated by RAS-activating mutations. Genomic analyses have recently described JMML mutational landscape; however, the nature of JMML-propagating cells (JMML-PCs) and the clonal architecture of the disease remained until now elusive. Combining genomic (exome, RNA-seq), Colony forming assay and xenograft studies, we detect the presence of JMML-PCs that faithfully reproduce JMML features including the complex/nonlinear organization of dominant/minor clones, both at diagnosis and relapse. Further integrated analysis also reveals that although the mutations are acquired in hematopoietic stem cells, JMML-PCs are not always restricted to this compartment, highlighting the heterogeneity of the disease during the initiation steps. We show that the hematopoietic stem/progenitor cell phenotype is globally maintained in JMML despite overexpression of CD90/THY-1 in a subset of patients. This study shed new lights into the ontogeny of JMML, and the identity of JMML-PCs, and provides robust models to monitor the disease and test novel therapeutic approaches.

Funder

Kay Kendall Leukemia Fund

ITMO Cancer

Ligue contre le cancer; Canceropole Ile de France, SFCE, Enfant et santé.

Cancer Research UK

Wellcome Trust

Publisher

Springer Science and Business Media LLC

Subject

Oncology,Cancer Research,Hematology

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