High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR

High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

Published:2018-05-21 Issue:6 Volume:36 Page:540-546
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Guo Xiaoge,Chavez Alejandro,Tung Angela,Chan Yingleong,Kaas Christian,Yin Yi,Cecchi Ryan,Garnier Santiago Lopez^ORCID,Kelsic Eric D^ORCID,Schubert Max,DiCarlo James E,Collins James J,Church George M^ORCID

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://www.nature.com/articles/nbt.4147.pdf

Reference48 articles.

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2. Ben-Shitrit, T. et al. Systematic identification of gene annotation errors in the widely used yeast mutation collections. Nat. Methods 9, 373–378 (2012).

3. DiCarlo, J.E. et al. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 41, 4336–4343 (2013).

4. Garst, A.D. et al. Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering. Nat. Biotechnol. 35, 48–55 (2017).

5. Bao, Z. et al. Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae. ACS Synth. Biol. 4, 585–594 (2015).

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