Fine mapping of the Cepaea nemoralis shell colour and mid-banded loci using a high-density linkage map

Author:

Johansen MargretheORCID,Saenko Suzanne,Schilthuizen MennoORCID,Berriman Matthew,Durbin Richard,Lawniczak Mara,Reeks Sarah,Howe Kerstin,Holroyd Nancy,McKenna Victoria,Niu Haoyu,Platte Radka,Howard Caroline,Amaral Raquel,Griffiths Andy,Mbye Haddijatou,Oatley Graeme,Prestwood Liam,Sampaio Filipa,Sheerin Edel,Strickland Michelle,Todorovic Maja,McCarthy Shane A.,Krasheninnikova Ksenia,Uliano-Silva Marcela,Wood Jonathan,Chow Will,Collins Joanna,Eldridge Camilla,Paulini Michael,Pelan Sarah,Pointon Damon-Lee,Sims Ying,Torrance James,Tracey Alan,Chapman Nikki,Calnan Sinead,Haug Ken,Heathcote Robina,Leech Chloe,Monaghan Jack,Muffato Matthieu,Potter Sophie,Ramona Lauma,Threlfall Jonathan,Varley Andrew,Vishwakumar Amit,Brown Max,Challis Rich,Gonzalez Pablo,Kieninger Manuela,King Erna,Kumar Sujai,Laumer Chris,Stevens Lewis,Vancaester Emmelien,Weber Claudia,Aunin Eerik,Reid Adam,Blaxter MarkORCID,Davison AngusORCID, , , , , , , , , , ,

Abstract

AbstractMolluscs are a highly speciose phylum that exhibits an astonishing array of colours and patterns, yet relatively little progress has been made in identifying the underlying genes that determine phenotypic variation. One prominent example is the land snail Cepaea nemoralis for which classical genetic studies have shown that around nine loci, several physically linked and inherited together as a ‘supergene’, control the shell colour and banding polymorphism. As a first step towards identifying the genes involved, we used whole-genome resequencing of individuals from a laboratory cross to construct a high-density linkage map, and then trait mapping to identify 95% confidence intervals for the chromosomal region that contains the supergene, specifically the colour locus (C), and the unlinked mid-banded locus (U). The linkage map is made up of 215,593 markers, ordered into 22 linkage groups, with one large group making up ~27% of the genome. The C locus was mapped to a ~1.3 cM region on linkage group 11, and the U locus was mapped to a ~0.7 cM region on linkage group 15. The linkage map will serve as an important resource for further evolutionary and population genomic studies of C. nemoralis and related species, as well as the identification of candidate genes within the supergene and for the mid-banding phenotype.

Publisher

Springer Science and Business Media LLC

Subject

Genetics (clinical),Genetics

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