Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids

Author:

Gu Wei,Deng Xianding,Lee Marco,Sucu Yasemin D.,Arevalo Shaun,Stryke Doug,Federman Scot,Gopez Allan,Reyes Kevin,Zorn KelseyORCID,Sample Hannah,Yu Guixia,Ishpuniani Gurpreet,Briggs Benjamin,Chow Eric D.ORCID,Berger Amy,Wilson Michael R.ORCID,Wang Candace,Hsu Elaine,Miller Steve,DeRisi Joseph L.,Chiu Charles Y.ORCID

Funder

Abbott Laboratories

U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute

U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases

U.S. Department of Health & Human Services | National Institutes of Health

Burroughs Wellcome Fund

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference65 articles.

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2. Rea, B., Maisel, J. R., Glaser, L. & Alby, K. Identification of clinically relevant mycobacterial species after extended incubation times in the BACTEC MGIT System. Am. J. Clin. Pathol. 151, 63–67 (2019).

3. Janda, J. M. & Abbott, S. L. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J. Clin. Microbiol. 45, 2761–2764 (2007).

4. Petti, C. A., Polage, C. R. & Schreckenberger, P. The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. J. Clin. Microbiol. 43, 6123–6125 (2005).

5. Reuwer, A. Q., van den Bijllaardt, W., Murk, J. L., Buiting, A. G. M. & Verweij, J. J. Added diagnostic value of broad-range 16S PCR on periprosthetic tissue and clinical specimens from other normally sterile body sites. J. Appl. Microbiol. 126, 661–666 (2019).

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