Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions-Reference-Cited by-同舟云学术

Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions

Published:2015-06-01 Issue:1 Volume:5 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Graewert Melissa A.,Franke Daniel,Jeffries Cy M.,Blanchet Clement E.,Ruskule Darja,Kuhle Katja,Flieger Antje,Schäfer Bernd,Tartsch Bernd,Meijers Rob,Svergun Dmitri I.

Abstract

Abstract Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/srep10734.pdf

Reference22 articles.

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