System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep38951.pdf
Reference34 articles.
1. Bustin, S. A., Benes, V., Nolan, T. & Pfaffl, M. W. Quantitative real-time RT-PCR–a perspective. J Mol Endocrinol 34, 597–601 (2005).
2. Zeka, F. et al. Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples. Sci Rep 6, 21418 (2016).
3. Heid, C. A., Stevens, J., Livak, K. J. & Williams, P. M. Real time quantitative PCR. Genome Res 6, 986–94 (1996).
4. Rutledge, R. G. Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications. Nucleic Acids Res 32, e178 (2004).
5. Tichopad, A., Dilger, M., Schwarz, G. & Pfaffl, M. W. Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res 31, e122 (2003).
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