Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency-Reference-Cited by-同舟云学术

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency

Published:2018-04 Issue:4 Volume:50 Page:1-9
ISSN:1226-3613
Container-title:Experimental & Molecular Medicine
language:en
Short-container-title:Exp Mol Med

Author:

Jang Da Eun,Lee Jae Young,Lee Jae Hoon,Koo Ok Jae,Bae Hee Sook,Jung Min Hee,Bae Ji Hyun^ORCID,Hwang Woo Sung,Chang Yoo Jin,Lee Yoon Hoo,Lee Han Woong,Yeom Su Cheong^ORCID

Publisher

Springer Science and Business Media LLC

Subject

Clinical Biochemistry,Molecular Biology,Molecular Medicine,Biochemistry

Link

http://www.nature.com/articles/s12276-018-0037-x.pdf

Reference28 articles.

1. Singh, P., Schimenti, J. C. & Bolcun-Filas, E. A mouse geneticist’s practical guide to CRISPR applications. Genetics 199, 1–15 (2015).

2. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

3. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).

4. Li, D. et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat. Biotechnol. 31, 681–683 (2013).

5. Li, W., Teng, F., Li, T. & Zhou, Q. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat. Biotechnol. 31, 684–686 (2013).

Cited by 42 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Precise and adaptable knock-out generation in wheat thanks to a multi-stop cassette;2024-06-13

2. Health research in the era of artificial intelligence: Advances in gene-editing study;Medicine Plus;2024-06

3. Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome;STAR Protocols;2024-03

4. Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis;BMC Biology;2024-01-17

5. The Use of Gene Editing Technology to Introduce Targeted Modifications in Woody Plants;Interdisciplinary Biotechnological Advances;2024