Abstract
AbstractTraumatic brain injury (TBI) is a global cause of morbidity and mortality. Initial management and risk stratification of patients with TBI is made difficult by the relative insensitivity of screening radiographic studies as well as by the absence of a widely available, noninvasive diagnostic biomarker. In particular, a blood-based biomarker assay could provide a quick and minimally invasive process to stratify risk and guide early management strategies in patients with mild TBI (mTBI). Analysis of circulating exosomes allows the potential for rapid and specific identification of tissue injury. By applying acoustofluidic exosome separation—which uses a combination of microfluidics and acoustics to separate bioparticles based on differences in size and acoustic properties—we successfully isolated exosomes from plasma samples obtained from mice after TBI. Acoustofluidic isolation eliminated interference from other blood components, making it possible to detect exosomal biomarkers for TBI via flow cytometry. Flow cytometry analysis indicated that exosomal biomarkers for TBI increase in the first 24 h following head trauma, indicating the potential of using circulating exosomes for the rapid diagnosis of TBI. Elevated levels of TBI biomarkers were only detected in the samples separated via acoustofluidics; no changes were observed in the analysis of the raw plasma sample. This finding demonstrated the necessity of sample purification prior to exosomal biomarker analysis. Since acoustofluidic exosome separation can easily be integrated with downstream analysis methods, it shows great potential for improving early diagnosis and treatment decisions associated with TBI.
Funder
United States Department of Defense | U.S. Army
Foundation for the National Institutes of Health
National Science Foundation
United States Army Medical Research Acquisition Activity
Publisher
Springer Science and Business Media LLC
Subject
Electrical and Electronic Engineering,Industrial and Manufacturing Engineering,Condensed Matter Physics,Materials Science (miscellaneous),Atomic and Molecular Physics, and Optics
Reference50 articles.
1. Faul, M., Wald, M. M., Xu, L. & Coronado, V. G. Traumatic brain injury in the United States; emergency department visits, hospitalizations, and deaths, 2002–2006. http://www.cdc.gov/traumaticbraininjury/tbi_ed.html (Centers for Disease Control and Prevention, 2010).
2. Hyder, A. A., Wunderlich, C. A., Puvanachandra, P., Gururaj, G. & Kobusingye, O. C. The impact of traumatic brain injuries: a global perspective. NeuroRehabilitation 22, 341–353 (2007).
3. Wilson, L. et al. The chronic and evolving neurological consequences of traumatic brain injury. Lancet Neurol. 16, 813–825 (2017).
4. Coronado, V. G. et al. Surveillance for traumatic brain injury-related deaths; United States, 1997–2007. MMWR Surveill. Summ 60, 1–32 (2011).
5. Kim, J. J. & Gean, A. D. Imaging for the diagnosis and management of traumatic brain injury. Neurotherapeutics 8, 39–53 (2011).
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献