STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA-Reference-Cited by-同舟云学术

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STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA

Published:2013-08-11 Issue:9 Volume:10 Page:910-916
ISSN:1548-7091
Container-title:Nature Methods
language:en
Short-container-title:Nat Methods

Author:

Heller Iddo,Sitters Gerrit,Broekmans Onno D,Farge Géraldine,Menges Carolin,Wende Wolfgang,Hell Stefan W,Peterman Erwin J G,Wuite Gijs J L

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Link

http://www.nature.com/articles/nmeth.2599.pdf

Reference32 articles.

1. Cornish, P.V. & Ha, T. A survey of single-molecule techniques in chemical biology. ACS Chem. Biol. 2, 53–61 (2007).

2. Moerner, W.E. New directions in single-molecule imaging and analysis. Proc. Natl. Acad. Sci. USA 104, 12596–12602 (2007).

3. Neuman, K.C. & Nagy, A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat. Methods 5, 491–505 (2008).

4. Joo, C., Balci, H., Ishitsuka, Y., Buranachai, C. & Ha, T. Advances in single-molecule fluorescence methods for molecular biology. Annu. Rev. Biochem. 77, 51–76 (2008).

5. Candelli, A., Wuite, G.J.L. & Peterman, E.J.G. Combining optical trapping, fluorescence microscopy and micro-fluidics for single molecule studies of DNA-protein interactions. Phys. Chem. Chem. Phys. 13, 7263–7272 (2011).

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