Abstract
AbstractThe propensity of bacteria to grow collectively in communities known as biofilms and their ability to overcome clinical treatments in this condition has become a major medical problem, emphasizing the need for anti-biofilm strategies. Antagonistic microbial interactions have extensively served as searching platforms for antibiotics, but their potential as sources for anti-biofilm compounds has barely been exploited. By screening for microorganisms that in agar-set pairwise interactions could antagonize Escherichia coli’s ability to form macrocolony biofilms, we found that the soil bacterium Bacillus subtilis strongly inhibits the synthesis of amyloid fibers –known as curli-, which are the primary extracellular matrix (ECM) components of E. coli biofilms. We identified bacillaene, a B. subtilis hybrid non-ribosomal peptide/polyketide metabolite, previously described as a bacteriostatic antibiotic, as the effector molecule. We found that bacillaene combines both antibiotic and anti-curli functions in a concentration-dependent order that potentiates the ecological competitiveness of B. subtilis, highlighting bacillaene as a metabolite naturally optimized for microbial inhibition. Our studies revealed that bacillaene inhibits curli by directly impeding the assembly of the CsgB and CsgA curli subunits into amyloid fibers. Moreover, we found that curli inhibition occurs despite E. coli attempts to reinforce its protective ECM by inducing curli genes via a RpoS-mediated competition sensing response trigged by the threatening presence of B. subtilis. Overall, our findings illustrate the relevance of exploring microbial interactions not only for finding compounds with unknown and unique activities, but for uncovering additional functions of compounds previously categorized as antibiotics.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Microbiology,Biotechnology
Reference48 articles.
1. Hall-Stoodley, L., Costerton, J. W. & Stoodley, P. Bacterial biofilms: from the natural environment to infectious diseases. Nat. Rev. Microbiol. 2, 95–108 (2004).
2. Flemming, H. C. & Wingender, J. The biofilm matrix. Nat. Rev. Microbiol. 8, 623–633 (2010).
3. Pinto, R. M., Soares, F. A., Reis, S., Nunes, C. & Van Dijck, P. Innovative strategies toward the disassembly of the EPS matrix in bacterial biofilms. Front. Microbiol. 11, 952 (2020).
4. Larsen, P. et al. Amyloid adhesins are abundant in natural biofilms. Environ. Microbiol. 9, 3077–3090 (2007).
5. Salinas, N., Povolotsky, T. L., Landau, M. & Kolodkin-Gal, I. Emerging roles of functional bacterial amyloids in gene regulation, toxicity, and immunomodulation. Microbiol. Mol. Biol. Rev. 85, e00062–00020 (2020).
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