Abstract
AbstracttRNA-derived small RNAs (tsRNAs), including tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs), are small regulatory RNAs processed from mature tRNAs or precursor tRNAs. tRFs and tiRNAs play biological roles through a variety of mechanisms by interacting with proteins or mRNA, inhibiting translation, and regulating gene expression, the cell cycle, and chromatin and epigenetic modifications. The establishment and application of research technologies are important in understanding the biological roles of tRFs and tiRNAs. To study the molecular mechanisms of tRFs and tiRNAs, researchers have used a variety of bioinformatics and molecular biology methods, such as microarray analysis, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR); Northern blotting; RNA sequencing (RNA-seq); cross-linking, ligation and sequencing of hybrids (CLASH); and photoactivatable-ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP). This paper summarizes the classification, action mechanisms, and roles of tRFs and tiRNAs in human diseases and the related signal transduction pathways, targeted therapies, databases, and research methods associated with them.
Publisher
Springer Science and Business Media LLC
Reference102 articles.
1. Shen, Y. et al. Transfer RNA-derived fragments and tRNA halves: biogenesis, biological functions and their roles in diseases. J. Mol. Med. (Berl., Ger.).96, 1167–1176 (2018).
2. Zhu, L., Xie, Y. & Guo, J. The biological functions of tRNA-derived fragments and tRNA Halves, and their roles in the pathogenesis. Prog. Biochem. Biophysics.44, 565–572 (2017).
3. Zhu, L. et al. tRNA-derived fragments and tRNA halves: the new players in cancers. Cancer Lett.452, 31–37 (2019).
4. Couvillion, M. T., Sachidanandam, R. & Collins, K. A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo. Genes Dev.24, 2742–2747 (2010).
5. Cole, C. et al. Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. RNA15, 2147–2160 (2009).
Cited by
125 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献