Author:
Liu Chao,Li Xiaohui,Huang Qingyu,Zhang Min,Lei Tianyu,Wang Fuhao,Zou Wenxue,Huang Rui,Hu Xiaoyu,Wang Cong,Zhang Xiaoling,Sun Bing,Xing Ligang,Yue Jinbo,Yu Jinming
Abstract
AbstractRadiochemotherapy (RCT) is a powerful treatment for cervical cancer, which affects not only malignant cells but also the immune and stromal compartments of the tumor. Understanding the remodeling of the local ecosystem induced by RCT would provide valuable insights into improving treatment strategies for cervical cancer. In this study, we applied single-cell RNA-sequencing to paired pre- and post-RCT tumor biopsies from patients with cervical cancer and adjacent normal cervical tissues. We found that the residual population of epithelial cells post-RCT showed upregulated expression of MHC class II genes. Moreover, RCT led to the accumulation of monocytic myeloid-derived suppressor cells with increased pro-inflammatory features and CD16+ NK cells with a higher cytotoxic gene expression signature. However, subclusters of T cells showed no significant increase in the expression of cytotoxic features post-RCT. These results reveal the complex responses of the tumor ecosystem to RCT, providing evidence of activation of innate immunity and MHC-II upregulation in cervical cancer.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Shandong Province
Publisher
Springer Science and Business Media LLC
Cited by
41 articles.
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