Abstract
AbstractRapid and ongoing climate change increases global temperature, impacts feeding, and reproduction in insects. The olfaction plays an important underlying role in these behaviors in most insect species. Here, we investigated how changing temperatures affect odor detection and ensuing behavior in three drosophilid flies: Drosophila novamexicana, D. virilis and D. ezoana, species adapted to life in desert, global, and subarctic climates, respectively. Using a series of thermal preference assays, we confirmed that the three species indeed exhibit distinct temperature preferences. Next, using single sensillum recording technique, we classified olfactory sensory neurons (OSNs) present in basiconic sensilla on the antenna of the three species and thereby identified ligands for each OSN type. In a series of trap assays we proceeded to establish the behavioral valence of the best ligands and chose guaiacol, methyl salicylate and isopropyl benzoate as representatives of a repellent, attractant and neutral odor. Next, we assessed the behavioral valence of these three odors in all three species across a thermal range (10-35 °C), with flies reared at 18 °C and 25 °C. We found that both developmental and experimental temperatures affected the behavioral performance of the flies. Our study thus reveals temperature-dependent changes in odor-guided behavior in drosophilid flies.
Funder
Max-Planck-Gesellschaft
Max Planck Center “Next Generation Insect Chemical Ecology
Publisher
Springer Science and Business Media LLC
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference50 articles.
1. Hansson, B. S. & Stensmyr, M. C. Evolution of Insect Olfaction. Neuron 72, 698–711 (2011).
2. Shanbhag, S. R. Atlas of olfactory organs of Drosophila melanogaster 1. Types, external organization, innervation and distribution of olfactory sensilla. Int. J. Insect. Morphol. Embryol. 21 (1999).
3. de Bruyne, M., Clyne, P. J. & Carlson, J. R. Odor Coding in a Model Olfactory Organ: The Drosophila Maxillary Palp. J. Neurosci. 19, 4520–4532 (1999).
4. Couto, A., Alenius, M. & Dickson, B. J. Molecular, Anatomical, and Functional Organization of the Drosophila Olfactory System. Curr. Biol. 15, 1535–1547 (2005).
5. Hallem, E. A. & Carlson, J. R. Coding of Odors by a Receptor Repertoire. Cell 125, 143–160 (2006).