Effect of nonsense-mediated mRNA decay factor SMG9 deficiency on premature aging in zebrafish-Reference-Cited by-同舟云学术

Effect of nonsense-mediated mRNA decay factor SMG9 deficiency on premature aging in zebrafish

Published:2024-05-28 Issue:1 Volume:7 Page:
ISSN:2399-3642
Container-title:Communications Biology
language:en
Short-container-title:Commun Biol

Author:

Lai Shaohong,Shiraishi Hiroshi,Sebastian Wulan Apridita,Shimizu Nobuyuki^ORCID,Umeda Ryohei,Ikeuchi Mayo,Kiyota Kyoko,Takeno Takashi,Miyazaki Shuya,Yano Shinji,Shimada Tatsuo,Yoshimura Akihiko,Hanada Reiko,Hanada Toshikatsu^ORCID

Abstract

AbstractSMG9 is an essential component of the nonsense-mediated mRNA decay (NMD) machinery, a quality control mechanism that selectively degrades aberrant transcripts. Mutations in SMG9 are associated with heart and brain malformation syndrome (HBMS). However, the molecular mechanism underlying HBMS remains unclear. We generated smg9 mutant zebrafish (smg9oi7/oi7) that have a lifespan of approximately 6 months or longer, allowing for analysis of the in vivo function of Smg9 in adults in more detail. smg9oi7/oi7 zebrafish display congenital brain abnormalities and reduced cardiac contraction. Additionally, smg9oi7/oi7 zebrafish exhibit a premature aging phenotype. Analysis of NMD target mRNAs shows a trend toward increased mRNA levels in smg9oi7/oi7 zebrafish. Spermidine oxidase (Smox) is increased in smg9oi7/oi7 zebrafish, resulting in the accumulation of byproducts, reactive oxygen species, and acrolein. The accumulation of smox mRNA due to NMD dysregulation caused by Smg9 deficiency leads to increased oxidative stress, resulting in premature aging.

Funder

MEXT | Japan Society for the Promotion of Science

Takeda Science Foundation

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s42003-024-06356-6.pdf

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